By John A. Lindbo (auth.), John M. Watson, Ming-Bo Wang (eds.)
Studies with regards to pathogen-mediated virus resistance in vegetation have been instrumental in delivering many of the ancient observations which eventually resulted in the very important discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has on account that revolutionized examine on plant-virus interactions. In Antiviral Resistance in crops: equipment and Protocols, specialist researchers within the box element the various equipment that are now established to check the phenomenon of RNA silencing in terms of viral infections of crops. those comprise tools and methods for the isolation and quantitative/qualitative analyses of plant small 21-24 nucleotide RNAs equivalent to small interfering RNAs (siRNAs) and microRNAs (miRNAs) in addition to the research and manipulation of virus-induced gene silencing (VIGS) in either monocotyledonous and dicotyledenous crops and using hairpin RNA (hpRNA) transgenes. Written within the hugely winning Methods in Molecular Biology™ sequence layout, chapters contain introductions to their respective themes, lists of the required fabrics and reagents, step by step, without difficulty reproducible laboratory protocols, and key tips about troubleshooting and keeping off recognized pitfalls.
Authoritative and functional, Antiviral Resistance in vegetation: equipment and Protocols seeks to assist scientists within the additional research of this crucially vital botanical trait.
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